tag:blogger.com,1999:blog-65314863265420247612010-01-09T13:51:39.204-08:00Fórmula Articular InternacionalDr. Boncihttp://www.blogger.com/profile/16655372154135233606andrew@drbonci.comBlogger1125tag:blogger.com,1999:blog-6531486326542024761.post-32438565686812708912008-07-14T19:05:00.001-07:002008-07-14T19:05:52.514-07:00Osteoarthritis Cartilage. 2007 Nov;15(11):1249-55.<br /><br /><span style="font-weight: bold;">Avocado soybean unsaponifiables (ASU) suppress TNF-alpha, IL-1beta, COX-2, iNOS </span><span style="font-weight: bold;">gene expression, and prostaglandin E2 and nitric oxide production in articular </span><span style="font-weight: bold;">chondrocytes and monocyte/macrophages.</span><br /><br />Au RY, Al-Talib TK, Au AY, Phan PV, Frondoza CG.<br /><br />OBJECTIVE: To evaluate the effects of avocado soybean unsaponifiables (ASU) on proinflammatory mediators in chondrocytes and monocyte/macrophage-like cells. DESIGN: To determine the dose response of ASU, chondrocytes (5 x 10(5) cells/well) were incubated at 5% CO(2), 37 degrees C for 72 h with (1) control media alone or (2) ASU at concentrations of 0.3, 0.9, 2.7, 8.3, and 25 microg/ml. Cells were activated with 20 ng/ml lipopolysaccharide (LPS) for 24 h and cell supernatants were analyzed for prostaglandin E(2) (PGE(2)) and nitrite content. Chondrocytes and THP-1 monocyte/macrophages (5 x 10(5) cells/well) were incubated at 5% CO(2), 37 degrees C for 72 h with (1) control media alone or (2) ASU (25 mug/ml). One set of cells was activated for 1 h with LPS (20 ng/ml) for both reverse-transcriptase PCR and real-time PCR analysis of tumor necrosis factor-alpha (TNF-alpha), interleukin-1-beta (IL-1beta), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) expression. One set of cells was activated for 24 h to analyze secreted PGE(2) and nitrite levels in the cellular supernatant. RESULTS: ASU reduced TNF-alpha, IL-1beta, COX-2, and iNOS expression in LPS-activated chondrocytes to levels similar to nonactivated control levels. The suppression of COX-2 and iNOS expression was paralleled by a significant reduction in PGE(2) and nitrite, respectively, in the cellular supernatant. ASU also reduced TNF-alpha and IL-1beta expression in LPS-activated monocyte/macrophage-like cells. CONCLUSION: The present study demonstrates that the anti-inflammatory activity of ASU is not restricted to chondrocytes, but also affects monocyte/macrophage-like cells that serve as a prototype for macrophages in the synovial membrane. These observations provide a scientific rationale for the pain-reducing and anti-inflammatory effects of ASU observed in osteoarthritis patients.<br /><br />PMID: 17845860 [PubMed - indexed for MEDLINE]<div class="blogger-post-footer"><img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/6531486326542024761-3243856568681270891?l=www.lubricaya.com' alt='' /></div>Dr. Boncihttp://www.blogger.com/profile/16655372154135233606andrew@drbonci.com0